You can add DE at any time. But test it first: if it blows through the filter and out the returns, you need to work on filter (slowing flow, adding sand) before adding more.
Buying small amounts of DE is hard, and if you want to clean up a cloudy pool, it may end up taking quite a bit anyhow, since filter runs may be very short. If you store the leftovers in a covered plastic bucket, it will last indefinitely.
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